Journal: Journal of Cellular and Molecular Medicine

Article Title: Ring finger protein 19A is overexpressed in non‐small cell lung cancer and mediates p53 ubiquitin‐degradation to promote cancer growth

doi: 10.1111/jcmm.16674

Figure Lengend Snippet: RNF19A interacts with p53 and promotes p53 ubiquitination. A, RNF19A‐silenced A549 and H460 cells were treated with MG132 and DMSO (control). RNF19A and p53 expression levels in the treated cells were detected via Western blotting. B, RNF19A‐silenced A549 and H460 cells were treated with CHX at the indicated time points. RNF19A and p53 expression levels in the treated cells were detected via Western blotting and the half‐life of p53 was calculated. C, Endogenous p53 and RNF19A form a protein complex in A549 cells. D, RNF19A co‐immunoprecipitates with p53 in A549 cells transfected with p53 and FLAG‐tagged RNF19A. E, A549 cells transfected with RNF19A expression plasmid or the RNF19A siRNA along with HA‐ubiquitin (Ub). Levels of p53 ubiquitination were detected via immunoprecipitation using the anti‐p53 antibody, followed by anti‐HA immunoblotting

Article Snippet: Briefly, the cell lysates were blocked with 40 μl Protein A + G agarose beads (Beyotime Biosciences) at 4°C for 2 hours and immunoprecipitated with 4‐10 μg mouse primary anti‐p53 (#60283‐2‐Ig, Proteintech), mouse primary anti‐FLAG (#HT201‐01, TransGen Biotech) or rabbit primary anti‐p53 (#10442‐1‐AP, Proteintech) antibodies overnight at 4°C.

Techniques: Expressing, Western Blot, Transfection, Plasmid Preparation, Immunoprecipitation

Journal: iScience

Article Title: Hepatitis C virus hypervariable region 1 antibodies interrupt E2-SR-B1 interaction to suppress viral infection

doi: 10.1016/j.isci.2023.106421

Figure Lengend Snippet: Figure 4

Article Snippet: FLAG tag (MA4) Mouse Monoclonal Antibody , Ray Antibody Biotech , Cat# RM1002.

Techniques: FLAG-tag, Recombinant, Bicinchoninic Acid Protein Assay, Western Blot, Derivative Assay, Software